FLP/frt system Spred1 gene floxed mice. Exon 7 was flanked by loxP sites. Immunology and Inflammation Research Developed by Akihiko Yoshimura, Keio University School of Medicine. A neo cassette flanked by FRT sites was removed by Flp-mediated recombination. 条件を付加する。<br>研究成果の公表にあたって寄託者の指定する文献を引用する。Genes Cells., 16, 803-814 (2011).<br>研究成果の公表にあたって謝辞の表明を必要とする。 吉村　昭彦 Akihiko YOSHIMURA In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Genes Cells., 16, 803-814 (2011).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. Spred1 floxed, B6-Spred1 floxed Spred1 floxed, B6-Spred1 floxed 慶應義塾大学医学部・吉村昭彦先生。Neoカセットはflp-mediated recombinationにより除去済み。 Cre/loxP system RBRC05760 Bacteriophage P1 loxP sites, yeast FRT (flipase recombination target) site, mouse Spred1 genomic DNA Spred1遺伝子のfloxedマウス。エクソン7がloxP配列で挟まれている。 Necessary documents for ordering:<ol><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: MTA for distribution with RIKEN BRC (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li></ol> true Cell Biology Research <a href='https://brc.riken.jp/mus/pcr05760'>Genotyping protocol -PCR-</a> Homozygote x Homozygote [or Crossing to C57BL/6JJcl] Homozygote x Homozygote [or Crossing to C57BL/6JJcl] B6.Cg-Spred1<tm1.1Ayos> B6.Cg-Spred1<tm1.1Ayos> C（3〜6か月） C (3-6 months)